Whole Genome Sequencing of MTB (SPIT SEQ) with DNA extraction-NGS
Also Known As
Test Parameters Included
- Mycobacterium tuberculosis Whole Genome Sequence Data (NGS-based) - pathogen identification and drug resistance mutation profiling
- Pathologist Molecular Genetics Remark - mapped against MTB drug resistance genotype-phenotype correlation database
Department
Methodology
Sample Required
Preparation
Schedule Report
28 Days
Emergency Report
Yes
Frequently Asked Questions
What does Whole Genome Sequencing of MTB detect?
It sequences the complete genome of Mycobacterium tuberculosis from sputum to identify the organism and detect genetic mutations associated with drug resistance, including to rifampicin, isoniazid, and second-line anti-TB drugs.
Is fasting required for this test?
No, fasting is not required. This is a molecular test performed on sputum samples, so dietary intake does not affect results.
What sample is needed for SPIT SEQ MTB?
Sputum sample is collected, and DNA is extracted prior to NGS library preparation. Adequate sputum volume ensures sufficient MTB DNA for sequencing.
How long does the test take?
The turnaround time is typically 5 to 7 working days, as NGS library preparation, sequencing, and bioinformatic analysis are time-intensive processes.
Can this test detect drug-resistant TB?
Yes, WGS identifies known resistance-associated mutations in genes such as rpoB, katG, rrs, and others, enabling comprehensive drug resistance profiling beyond conventional culture-based methods.
Who should undergo this test?
It is indicated for suspected or confirmed TB patients, especially those with treatment failure, relapse, or suspected multidrug-resistant and extensively drug-resistant TB, to guide personalized anti-TB therapy.
Test Description
Test Code
MOG218
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.