Whole Genome Sequencing of MTB Shotgun with DNA Extraction - NGS
Also Known As
Test Parameters Included
- Mycobacterium tuberculosis Whole Genome Sequence with Drug Resistance Mutation Profile
- Pathologist Molecular Genetics Remark - mapped against WHO-recommended TB drug resistance gene catalog
Department
Methodology
Sample Required
Preparation
* Over-the-Counter Medication Effects: Not applicable, as this is a microbial genomic test. Patient medications do not directly affect the bacterial DNA sequencing. *
* Clinical History Required (Absolutely Crucial): Providing detailed and accurate clinical information is paramount for the meaningful interpretation of the WGS results, especially regarding drug resistance and transmission patterns. * Patient Details: Age, gender, previous TB history (e.g., new case, relapse, treatment failure), treatment regimen (current and past anti-TB drugs), and treatment outcomes. *
* TB Diagnosis Details: Date of TB diagnosis, site of infection (e.g., pulmonary, extra-pulmonary like lymph node, bone, CNS), and results of conventional phenotypic drug susceptibility testing (DST), if available. *
* Previous Molecular Test Results: Results of any prior molecular tests for TB (e.g., GeneXpert MTB/RIF, TB-PCR, Line Probe Assay (LPA)), particularly if they indicated Rifampicin resistance or other specific mutations. *
* Travel History & Exposure: Relevant travel history, exposure to known TB cases (especially DR-TB cases), or residence in high TB burden areas. *
* HIV Status & Co-morbidities: Information on HIV status and other co-morbidities that might affect TB treatment response or immune status. *
* Reason for WGS Request: Clearly state the primary reason for requesting WGS (e.g., suspected drug resistance, treatment failure, contact investigation, epidemiological surveillance, atypical presentation). *
* Sample Collection & Transport Guidelines: * For Culture Isolates: Ensure the MTB isolate is pure, viable, and grown to sufficient density. Follow strict biosafety level (BSL-3) procedures for handling and packaging. Transport according to UN 3373 (Biological Substance, Category B) guidelines, typically at ambient temperature for short durations or refrigerated/frozen for longer transport. *
* For Clinical Samples (TB Positive Report): The sample should be a primary clinical specimen (e.g., sputum, BAL, CSF) that has tested positive for M. tuberculosis complex by a sensitive molecular method (e.g., GeneXpert, TB PCR). The sample should be handled and transported according to infectious substance guidelines. It is crucial to have a sufficient bacterial load in the sample for successful direct WGS; culture isolates are generally preferred for higher success rates. *
* Consultation with Experts: * It is advisable to consult with an infectious disease specialist or microbiologist for appropriate sample selection and interpretation of results, especially in complex DR-TB cases.
Schedule Report
28 Days
Emergency Report
Yes
Frequently Asked Questions
What does Whole Genome Sequencing of MTB detect?
It sequences the complete genome of Mycobacterium tuberculosis from culture isolates, identifying all drug resistance mutations across genes like rpoB, katG, rpsL, and gyrA in a single test.
What sample is required for this test?
Mycobacterium tuberculosis culture isolates from sputum, tissue, or other clinical specimens. Only culture-positive TB samples are accepted for WGS analysis.
How is DNA extracted for shotgun sequencing?
Genomic DNA is extracted from the MTB culture isolate using standardized lysis and purification methods, then fragmented for shotgun NGS library preparation and sequencing.
What is the turnaround time for MTB WGS results?
Reports are typically available within 5–7 working days, as WGS requires culture confirmation, DNA extraction, sequencing, and bioinformatics analysis.
Can this test detect both drug-sensitive and drug-resistant TB?
Yes, WGS identifies the full drug resistance profile including MDR, XDR, and pre-XDR patterns by detecting mutations in first-line and second-line TB drug resistance genes.
Is this test available at Dr. Patho Labs?
Yes, Dr. Patho Labs in Chhatrapati Sambhajinagar offers MTB Whole Genome Sequencing with DNA Extraction using NGS as a NABL ISO accredited molecular genetics test.
Test Description
Test Code
MOG206
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