Test

Hepatitis B Virus DNA HBV - Qualitative PCR EDTA

₹5,625 ₹4,800

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Also Known As

#1) Qualitative HBV DNA Molecular Detection Array #2) Hepatitis B Nucleic Acid Amplification Test (NAAT) Screen #3) Early Window Period HBV Genomic DNA Assay #4) High-Sensitivity Qualitative Hepatitis B PCR Test #5) Core HBV Viral DNA Plasma Matrix #6) Kinetic EDTA Plasma HBV Molecular Sentinel #7) Occult Hepatitis B Infection (OBI) Detection Screen #8) Targeted Pre-Core & Surface Gene HBV DNA Study #9) Executive Post-Exposure HBV Molecular Triage #10) Microvascular Hepatic Viral Nucleic Acid Detection Grid #11) Acute & Chronic Viral Hepatitis B Differential #12) Absolute Bio-Genomic HBV Viral DNA Mass Review #13) PathoFit Primary Viral Molecular Care Package #14) Absolute Circulating HBV Genome Asset Review #15) Standard Multi-Platform Real-Time PCR HBV Protocol

Test Parameters Included

HBV DNA Qualitative Detection - Direct molecular amplification targeting conserved regions of the HBV genome (such as the overlapping S and P gene regions or the core gene) to confirm the presence (Detected) or absence (Not Detected) of active circulating viral DNA.
Internal Control Amplification - Validates the efficiency of automated nucleic acid extraction and confirms the absence of enzymatic PCR inhibitors in the plasma sample, safeguarding against false-negative reports.
Molecular Pathologist Remarks - Detailed medical interpretation correlating the qualitative PCR finding with clinical history, serological markers (HBsAg, HBeAg), and recommended next steps.

Department

MOLECULAR BIOLOGY

Methodology

PCR : REAL-TIME POLYMERASE CHAIN REACTION (REAL-TIME PCR) The EDTA plasma specimen is processed using automated Real-Time Polymerase Chain Reaction (qPCR): * Plasma Separation: Whole blood collected in EDTA is centrifuged under standardized protocols to isolate clear, cell-free plasma. * Automated DNA Extraction & Purification: Viral particles are lysed using chemical lysis buffers and proteinase enzymes. Magnetic silica beads bind the released viral DNA, followed by automated washing steps to remove cellular debris, proteins, and chemical inhibitors. * Target Amplification: Sequence-specific forward and reverse primers target highly conserved sequences within the HBV genome. Thermostable DNA polymerase amplifies the target segment through repetitive thermal cycling (denaturation, annealing, and extension). * Fluorogenic Probe Detection: Dual-labeled fluorescent hydrolysis probes (TaqMan chemistry) bind specifically between the primers. As the DNA strand is synthesized, the 5'-to-3' exonuclease activity of the polymerase cleaves the probe, releasing a reporter dye signal. * Real-Time Signal Acquisition: Optical sensors track the exponential accumulation of fluorescence cycle-by-cycle (C_t value), delivering high analytical specificity and a limit of detection capable of identifying as few as 10 to 20 International Units per milliliter (IU/mL) of HBV DNA.

Sample Required

WB-EDTA PLASMA (3ML)

Preparation

CRITICAL PREPARATION & SAMPLE LOGISTICS: * No Fasting Required: Strict fasting is entirely unnecessary. Routine meals, fluid intake, and daily non-antiviral medications do not alter circulating viral nucleic acids. * Antiviral Medication Documentation: Inform the laboratory and your treating clinician if you are currently taking or have recently discontinued nucleoside/nucleotide analogue antiviral therapy (e.g., Tenofovir, Entecavir, Lamivudine). Potent antivirals suppress circulating viral DNA below the analytical detection threshold, leading to a "Not Detected" result despite past infection. * Sample Required: Requires 3 mL of venous blood collected cleanly into a purple-top EDTA tube. Plasma must be separated and kept refrigerated at 2–8^\circ\text{C} or frozen to preserve viral DNA stability. * Avoid Heparin Tubes: Heparin is a potent enzymatic inhibitor of DNA polymerase and causes PCR assay failure. Only EDTA is acceptable.

Schedule Report

24 hours

Emergency Report

Yes

Frequently Asked Questions

What is HBV DNA qualitative PCR used for?

It detects the presence or absence of Hepatitis B virus genetic material in blood, confirming active viral replication even when HBsAg is borderline or unclear.

Is fasting required for this test?

No, fasting is not required. Blood can be collected at any time of day.

What sample is used for HBV DNA PCR?

Whole blood in EDTA tube or plasma is used. The sample is processed to extract viral DNA for amplification.

How long does it take to get results?

Reports are typically available within 24 to 48 hours via www.drpatholab.in or WhatsApp/Email.

What does a positive HBV DNA result mean?

A positive result indicates active Hepatitis B viral replication in the body, confirming ongoing infection that may require antiviral treatment and monitoring.

Can this test detect early Hepatitis B infection?

Yes, HBV DNA can become detectable before HBsAg appears, making it useful for early diagnosis and identifying window-period infections.

Test Description

Hepatitis B Virus DNA HBV - Qualitative PCR EDTA Examination A Hepatitis B Virus (HBV) DNA Qualitative Test detects the presence of the Hepatitis B v...

Test Code

H18

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