Desmoglein Marker
Also Known As
Test Parameters Included
- Desmoglein-1 and/or Desmoglein-3 IHC Expression Score (positive/negative staining pattern in epidermal/ mucosal tissue)
- Pathologist Histopathology Remark - correlated with clinical suspicion of pemphigus group disorders
Department
Methodology
Sample Required
Preparation
* The primary sample required for this test is a Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Block. This is a preserved block of tissue that was obtained from your previous biopsy or surgical procedure.
2. Clinical History and Previous Reports:
* It is crucial to provide a copy of any previous histopathology reports for reference. These reports contain initial diagnoses and detailed descriptions of your tissue, which are invaluable for the pathologist.
* A complete clinical history is absolutely essential for the pathologist to accurately interpret the Desmoglein staining results within the correct context. This should include:
* The exact site of the biopsy or surgical procedure from where the tissue block was collected.
* Any provisional diagnosis or clinical suspicion your treating doctor has (e.g., suspected squamous cell carcinoma, undifferentiated tumor, or metastatic cancer of unknown primary).
* A brief summary of your relevant medical history and the reason your doctor ordered this specific test (e.g., presence of a mass in the lung, skin lesion, or a metastatic lesion whose origin is unclear).
* Information regarding any previous treatments (such as radiation or chemotherapy) that might affect the tissue's cellular composition or protein expression.
3. Tissue Block Quality:
* The FFPE block must contain sufficient viable tumor tissue to allow for cutting multiple thin sections necessary for the Desmoglein stain and any other complementary tests.
* The tissue must have been properly fixed in 10% neutral buffered formalin during its initial processing. Incorrect fixation can affect the quality of the proteins and potentially lead to inaccurate test results.
Schedule Report
5 Days
Emergency Report
Yes
Frequently Asked Questions
What does a Desmoglein Marker test detect?
It detects the presence and distribution of desmoglein-1 and desmoglein-3 proteins in tissue biopsies using immunohistochemistry, aiding diagnosis of autoimmune blistering diseases like pemphigus vulgaris and pemphigus foliaceus.
Why is this test performed on tissue instead of blood?
IHC on tissue sections reveals the exact staining pattern and location of desmoglein proteins, which helps differentiate pemphigus subtypes and distinguish them from other blistering conditions like bullous pemphigoid.
What sample is required for this test?
Tissue paraffin blocks (FFPE) are required, typically obtained via skin or mucosal biopsy from the affected area.
How long does the report take?
IHC reports are typically available within 3–5 working days from receipt of the paraffin block at the lab.
Can this test replace a blood autoantibody test?
No, it complements direct immunofluorescence and ELISA for desmoglein antibodies. Both tissue IHC and serological tests are often used together for definitive diagnosis.
Who should consult for this test?
Dermatologists and pathologists interpret these results in the context of clinical presentation to guide diagnosis and management of autoimmune blistering disorders.
Test Description
Test Code
D26
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