Vitamin D Total (D2 & D3) 25-OH
Also Known As
Test Parameters Included
- 25-Hydroxy Vitamin D Total:
This parameter represents the sum of Vitamin D2 and Vitamin D3. Because 25-Hydroxy Vitamin D has a long "half-life" (stays in the blood for several weeks), it is the best indicator of long-term Vitamin D status rather than the "active" form (1,25-Dihydroxy), which fluctuates daily.
Department
Methodology
Sample Required
Preparation
Schedule Report
12 Hours
Emergency Report
Yes
Frequently Asked Questions
Why is this test called "25-Hydroxy"?
It refers to the form of Vitamin D that is processed by the liver. It is the most stable form to measure in the blood.
Why does the report take 6 hours?
Our automated Chemiluminescence platform is designed for precision and efficiency, ensuring quality-checked results within the same day.
What is the difference between D2 and D3?
D2 (Ergocalciferol) mostly comes from plants/fortified foods, while D3 (Cholecalciferol) is made by your skin from sunlight and found in animal products. Both are vital.
Can I get enough Vitamin D from food?
It is difficult, as few foods are naturally rich in it. Sun exposure and supplements are often necessary.
Does sunscreen prevent Vitamin D production?
Yes, high-SPF sunscreen can block the UV rays needed for the skin to produce Vitamin D.
Can I drink water before the test?
Yes, plain water is fine and does not affect your vitamin levels.
Why is Vitamin D low in people with dark skin?
Higher melanin levels act as a filter, requiring more sun exposure to produce the same amount of Vitamin D as lighter skin.
Can weight affect Vitamin D levels?
Yes. Since it is fat-soluble, Vitamin D can get "trapped" in body fat, often leading to lower blood levels in obese individuals.
What is "Vitamin D Toxicity"?
It happens when very high doses of supplements cause too much calcium to build up in the blood, which can damage the heart and kidneys.
Does age affect the result?
Yes. As people age, their skin becomes less efficient at producing Vitamin D from sunlight.
Why must I stop Biotin?
Biotin can interfere with the lab's light-detection technology, making your Vitamin D levels look incorrectly high or low.
Is this test the same as a "Calcium test"?
No, but they are related. Vitamin D is the "key" that lets Calcium into your body.
Can Vitamin D affect my mood?
Yes. Low levels are often linked to Seasonal Affective Disorder (SAD) and general low mood.
How often should I get tested?
Usually once or twice a year, especially if you are taking supplements to correct a deficiency.
Is the test result confidential?
Absolutely. At Dr. Patho Labs & Diagnostics, all nutrient and wellness data are protected by strict privacy protocols.
Test Description
Test Code
H02
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