Molecular Genetic HLA -Typing High Resolution ( Donor ) - NGS
Test Code
MOG91
Also Known As
No data available
Test Parameters Included
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Department
MOLECULAR GENETICS
Methodology
NGS (Next-Generation Sequencing)
NGS is a revolutionary technology in molecular diagnostics that has become the gold standard for high-resolution HLA typing. It provides unparalleled detail and accuracy, overcoming the limitations of older methods like serology and lower-resolution molecular techniques. The detailed process is as follows:
* DNA Extraction: Genomic DNA is first extracted from the donor's whole blood sample. High-quality and sufficient quantity of DNA are critical for the success of the test.
* PCR Amplification: Specific regions of the HLA genes (e.g., HLA-A, -B, -C, -DRB1, -DQB1, -DPB1) are targeted and amplified using Polymerase Chain Reaction (PCR). These primers are designed to cover all known variations (alleles) of the HLA genes.
* Library Preparation: The amplified DNA fragments are prepared for sequencing. This involves fragmenting the DNA, ligating adapter sequences to the ends of the fragments, and indexing each sample with a unique barcode. This process allows multiple samples to be sequenced together in a single run.
* Massive Parallel Sequencing: The prepared DNA library is loaded onto a sequencing instrument (e.g., Illumina platform). The instrument sequences millions of small DNA fragments in parallel. This is where NGS gets its name—it sequences an entire panel of genes simultaneously, rather than one gene at a time.
* Bioinformatic Analysis: The massive amount of raw sequencing data is then analyzed using sophisticated bioinformatics software. The software aligns the sequenced fragments to a reference HLA database (e.g., the IPD-IMGT/HLA Database). This alignment allows for the precise determination of the specific nucleotide sequence of the HLA genes and the identification of the exact allele at each locus. The depth of sequencing provided by NGS is sufficient to resolve any sequence ambiguities and provide a complete, high-resolution genotype.
* Report Generation: The final genotype, expressed at the allele level (e.g., A*02:01:01:01), is generated and interpreted by an expert.
Sample Required
DONOR WB EDTA
* Preferred Sample: Whole Blood (WB) in a Lavender-top tube containing the anticoagulant EDTA (ethylenediaminetetraacetic acid). This is the gold standard for genetic testing as it provides a stable source of high-quality genomic DNA.
* Volume: A minimum of 3-5 mL of whole blood is typically required.
* Handling and Transport: The sample should be gently inverted to mix with the EDTA. It must be transported to the laboratory at refrigerated temperatures (2-8°C). The sample is stable for several days under these conditions, but prompt processing is always recommended. Freezing of the whole blood sample is generally not recommended as it can compromise DNA quality.
* Alternate Samples: In certain rare cases, other sources of DNA such as cheek swabs (buccal swabs) or bone marrow aspirate can be used, but whole blood is the most reliable for comprehensive and high-quality results.
Preparation
* No special preparation is required. Fasting is not necessary for this genetic test, as DNA is stable and not affected by food or drink. * No dietary or lifestyle restrictions are needed. * Timing: T... Read more
Schedule Report
15 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.