Molecular Genetic ACE Gene Polymorphism - PCR
Test Code
MOG53
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
PCR
This test primarily utilizes PCR (Polymerase Chain Reaction). For polymorphism detection, PCR can be combined with various downstream techniques, though the prompt specifically mentions "PCR," which typically implies a fragment size analysis or a method like Allele-Specific PCR or PCR-Restriction Fragment Length Polymorphism (RFLP) for identifying the specific variants. For the ACE I/D polymorphism, the most common approach is PCR followed by gel electrophoresis to distinguish the presence or absence of the insertion fragment.
* PCR Principle: PCR amplifies specific regions of the ACE gene that contain the known polymorphism (most commonly the Insertion/Deletion, or I/D, polymorphism). This amplification creates millions of copies of the target DNA sequence from a small initial sample.
* Polymorphism Detection (typically for ACE I/D): The amplified DNA fragments are then analyzed, usually by gel electrophoresis. The 'Insertion' (I) allele will result in a longer PCR product, while the 'Deletion' (D) allele will result in a shorter product. Individuals can be homozygous for the Insertion (II), homozygous for the Deletion (DD), or heterozygous (ID), each producing distinct band patterns on the gel.
This method allows for the accurate and reliable genotyping of the ACE I/D polymorphism.
Sample Required
Whole Blood (WB) in EDTA
* Typically, 2-5 mL of Whole Blood collected in an EDTA (lavender-top) vacutainer tube is required. EDTA prevents blood clotting and ensures the stability of DNA within white blood cells.
* Proper collection and timely transport (preferably refrigerated, not frozen) are crucial to ensure DNA integrity.
Preparation
Proper collection and handling of the blood sample are essential for accurate and reliable genetic testing. * No specific patient preparation is required (e.g., fasting or dietary restrictions). * Sam... Read more
Schedule Report
7 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
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