Molecular Genetic COL1A1 Gene Polymprphism - PCR
Test Code
MOG52
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
PCR / Sanger Sequencing
This test employs a combination of two molecular techniques:
* PCR (Polymerase Chain Reaction): This is used to amplify specific regions of the COL1A1 gene where known polymorphisms are located. PCR creates millions of copies of the target DNA sequence from a small initial sample, making it detectable.
* Sanger Sequencing: After PCR amplification, Sanger sequencing (also known as the "chain-termination method") is performed. This technique determines the exact order of nucleotides (A, T, C, G) in the amplified DNA fragment. By comparing the obtained sequence to a reference sequence, any variations or polymorphisms within the targeted region of the COL1A1 gene can be identified.
This combined approach allows for highly accurate and specific detection of genetic variations within the COL1A1 gene.
Sample Required
Whole Blood (WB) in EDTA
* Typically, 2-5 mL of Whole Blood collected in an EDTA (lavender-top) vacutainer tube is required. EDTA prevents blood clotting and preserves the DNA within white blood cells.
* Proper collection and timely transport (preferably refrigerated) are crucial to ensure DNA integrity.
Preparation
Proper collection and handling of the blood sample are essential for accurate and reliable genetic testing. * No specific patient preparation is required (e.g., fasting or dietary restrictions). * Sam... Read more
Schedule Report
7 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.