Molecular Genetic DNA Isolation And Storage
Test Code
MOG51
Also Known As
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Test Parameters Included
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Department
MOLECULAR GENETICS
Methodology
Spin Column / Salting Out (DNA Extraction)
This service involves the extraction and purification of high-quality DNA from various biological samples, typically using one of two primary methodologies:
* Spin Column Method: This is a widely used, rapid, and efficient method for DNA extraction. It involves cell lysis, binding of DNA to a silica-based membrane within a spin column in the presence of chaotropic salts, washing away contaminants (proteins, RNA, other cellular debris), and then eluting the purified DNA from the column using a low-salt buffer or water. This method yields highly pure DNA suitable for various downstream molecular applications.
* Salting Out Method: This is a classic, non-toxic, and relatively inexpensive method for DNA extraction. It involves cell lysis, followed by the precipitation of proteins and other contaminants using high concentrations of salt (e.g., sodium chloride, ammonium acetate). The DNA remains in solution and is then precipitated from the aqueous phase using ethanol or isopropanol. The precipitated DNA is then washed and rehydrated. This method can yield large amounts of high-quality DNA.
Both methods aim to provide purified DNA that is free from contaminants (proteins, RNA, inhibitors) and suitable for long-term storage and subsequent molecular genetic analyses.
Sample Required
POC/FFPE/AF/WB EDTA : POC (Products of Conception) / FFPE (Formalin-Fixed Paraffin-Embedded) / AF (Amniotic Fluid) / WB (Whole Blood) in EDTA
This indicates that DNA can be successfully isolated from a variety of biological sources, each requiring specific handling and processing protocols due to their unique properties:
* POC (Products of Conception): Tissue samples from miscarriages or abortions, often used for genetic analysis to determine the cause of pregnancy loss.
* FFPE (Formalin-Fixed Paraffin-Embedded) Tissue: Tissue samples routinely preserved for histopathological diagnosis. DNA extraction from FFPE is challenging due to cross-linking caused by formalin fixation but is crucial for retrospective genetic studies and tumor profiling.
* AF (Amniotic Fluid): Fluid collected during amniocentesis, containing fetal cells, used for prenatal genetic diagnosis.
* WB (Whole Blood) in EDTA: The most common and ideal source for germline DNA, collected in an EDTA (lavender-top) vacutainer tube to prevent clotting and preserve cellular integrity. This provides high-quality and quantity DNA.
STORAGE PERIOD AFTER ISOLATION: 2 Years
This indicates that the extracted and purified DNA will be stored by the laboratory for a period of up to 2 years from the date of isolation. Proper storage conditions (e.g., at -20°C or -80°C in nuclease-free water or buffer) are critical to maintain DNA integrity and stability for future testing or research purposes.
Preparation
The preparation for DNA isolation primarily involves the proper collection and handling of the biological sample. Patient preparation is generally minimal or depends on the specific sample type being ... Read more
Schedule Report
STORAGE PERIOD AFTER
Emergency Report
Yes
Frequently Asked Questions
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Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.