Molecular Genetic Warfarin ( CYP2C9*2, CYP2C9*3, VKORC1 ) by RT-PCR
Test Code
MOG33
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Real-Time Polymerase Chain Reaction) / PCR / Allele-Specific PCR / High-Resolution Melt Analysis (HRM)
For Warfarin pharmacogenomic testing, the specific method used is typically a DNA-based PCR technique designed to detect specific single nucleotide polymorphisms (SNPs). While the prompt mentions "RT-PCR," it's more accurately Real-Time PCR (which is DNA-based for SNP detection, not RNA-based Reverse Transcription PCR) or other PCR-based methods optimized for genotyping. The commonly employed methodologies include:
* Real-Time PCR (RT-PCR, in this context means "quantitative" or "real-time" PCR): This method uses fluorescent probes to detect the amplification of specific alleles in real-time. It's highly sensitive, fast, and can quantify DNA, making it suitable for high-throughput genotyping of known SNPs.
* Allele-Specific PCR: Uses primers designed to specifically amplify only one allele of a gene, allowing for the presence or absence of a specific SNP to be determined.
* High-Resolution Melt Analysis (HRM): A post-PCR technique that detects genetic variations based on characteristic DNA melting curves.
* Sanger Sequencing (for confirmation or less common variants): Can be used if a non-standard variant is suspected or for confirmatory purposes.
These methods are used to specifically detect common genetic variants (SNPs) in the CYP2C9 and VKORC1 genes that influence an individual's response to Warfarin.
Sample Required
Whole Blood (WB) in EDTA Vacutainer
(A sample of whole blood collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the blood sample for high-quality DNA extraction.)
Preparation
No specific preparation is required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood sample collection. * No Special Timing: Blood can be drawn at any time of day.... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
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