Molecular Genetic Multiple Ligation Probe Application - MLPA
Test Code
MOG31
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
MLPA (Multiplex Ligation-dependent Probe Amplification)
MLPA (Multiplex Ligation-dependent Probe Amplification) is a highly sensitive and robust molecular technique used for the detection of copy number variations (CNVs), which include gene deletions or duplications. It allows for the simultaneous detection of up to 50 different genomic sequences in a single reaction. The principle relies on the hybridization of specifically designed probes to target DNA sequences. Each probe consists of two oligonucleotides that, when bound adjacently to the target sequence, can be ligated. The ligated probes are then amplified by PCR using universal primers. The amount of each amplified product is directly proportional to the number of target DNA copies present in the sample. By comparing the peak heights or areas of the amplified fragments from a test sample to those from a normal reference sample, gains or losses of specific genomic regions (exons, entire genes, or chromosomal segments) can be accurately identified. MLPA is particularly valuable for detecting conditions caused by single or multiple exon deletions/duplications that are often missed by traditional Sanger sequencing or standard NGS panels which primarily focus on point mutations and small indels.
Sample Required
Whole Blood (WB) in EDTA Vacutainer
(A sample of whole blood collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the blood sample for high-quality DNA extraction.)
Preparation
No specific preparation is typically required for this molecular genetic test. * No Fasting Required: Fasting is not necessary for this blood sample collection. * No Special Timing: Blood can be drawn... Read more
Schedule Report
20 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.