Molecular Genetic Hemochromotosis Mutation - RT-PCR
Test Code
MOG14
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Reverse Transcription Polymerase Chain Reaction) / PCR / Allele-Specific PCR
The Molecular Genetic Hemochromatosis Mutation test primarily utilizes PCR (Polymerase Chain Reaction), often in the form of Allele-Specific PCR (AS-PCR). While the prompt mentions RT-PCR, this test directly analyzes DNA, not RNA, to detect specific genetic variations (mutations) within the HFE gene. PCR is used to amplify the regions of the HFE gene where the common mutations (C282Y and H63D) occur. AS-PCR uses primers specifically designed to bind only to the mutated DNA sequences, allowing for selective amplification of the mutated alleles. This molecular approach offers high sensitivity and specificity for identifying these key genetic alterations, which are crucial for the diagnosis of hereditary hemochromatosis (HH) and for carrier screening.
Sample Required
WB EDTA Whole Blood (WB) in EDTA Vacutainer
(A sample of whole blood collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the blood sample for DNA extraction.)
Preparation
No specific preparation is typically required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood sample collection. * No Special Timing: Blood can be drawn at any ti... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.