Molecular Genetic MPL - Myelo Proliferative Leukemia - RT-PCR
Test Code
MOG13
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Reverse Transcription Polymerase Chain Reaction) / PCR / Sanger Sequencing
The Molecular Genetic MPL - Myeloproliferative Leukemia test primarily employs PCR (Polymerase Chain Reaction) followed by Sanger Sequencing. While the prompt mentions RT-PCR, this test directly analyzes DNA, not RNA, to detect specific mutations within the MPL gene. PCR is used to amplify the relevant regions of the MPL gene (specifically exon 10), where the common activating mutations occur. Sanger Sequencing then provides the precise nucleotide sequence to identify specific point mutations (e.g., W515L/K/A/R). This molecular approach offers high sensitivity and specificity for identifying MPL mutations, which are crucial diagnostic markers for a subset of myeloproliferative neoplasms (MPNs), particularly Essential Thrombocythemia (ET) and Primary Myelofibrosis (PMF).
Sample Required
WB/BMA EDTA: Whole Blood (WB) or Bone Marrow Aspirate (BMA) in EDTA Vacutainer
(A sample of whole blood or bone marrow aspirate collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the sample for DNA extraction.)
Preparation
No specific preparation is typically required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood or bone marrow sample collection. * No Special Timing: Blood or bone... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.