Molecular Genetic Calreticulin CALR Gene Mutation Analysis - RT-PCR
Test Code
MOG12
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Reverse Transcription Polymerase Chain Reaction) / PCR / Fragment Analysis / Sanger Sequencing
The Molecular Genetic Calreticulin (CALR) Gene Mutation Analysis test primarily utilizes PCR (Polymerase Chain Reaction), followed by methods such as Fragment Analysis or Sanger Sequencing. While the prompt mentions RT-PCR, this test directly analyzes DNA, not RNA, to detect specific CALR gene mutations. PCR is used to amplify the region of the CALR gene (specifically exon 9) where the common frameshift mutations occur. Fragment analysis then detects changes in the length of the amplified DNA fragments, which correspond to the characteristic deletions or insertions (e.g., type 1/52bp deletion or type 2/5bp insertion). Alternatively, Sanger sequencing provides the precise nucleotide sequence to confirm the specific mutation type. This molecular approach offers high sensitivity and specificity for identifying CALR mutations, which are crucial diagnostic markers for a significant subset of myeloproliferative neoplasms (MPNs).
Sample Required
WB/BMA EDTA : Whole Blood (WB) or Bone Marrow Aspirate (BMA) in EDTA Vacutainer
(A sample of whole blood or bone marrow aspirate collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the sample for DNA extraction.)
Preparation
No specific preparation is typically required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood or bone marrow sample collection. * No Special Timing: Blood or bone... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.