Molecular Genetic JAK2 ( Exon 12-14 ) Mutation Analysis - RT-PCR
Test Code
MOG11
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Reverse Transcription Polymerase Chain Reaction) / PCR / High-Resolution Melting (HRM) / Sanger Sequencing
The Molecular Genetic JAK2 (Exon 12-14) Mutation Analysis test typically employs PCR (Polymerase Chain Reaction) combined with downstream detection methods such as High-Resolution Melting (HRM) analysis or Sanger Sequencing. While the prompt mentions RT-PCR, this test directly analyzes DNA, not RNA, to detect genetic mutations within the JAK2 gene. These PCR-based methods are highly sensitive and specific for identifying a range of mutations within exons 12 to 14 of the JAK2 gene, complementing the detection of the more common V617F mutation. PCR amplifies the target DNA regions, and then HRM or Sanger sequencing allows for precise identification of sequence variations. HRM detects changes in the DNA melting profile indicative of a mutation, while Sanger sequencing provides the exact nucleotide sequence for definitive mutation identification. This combined approach offers a comprehensive screen for less common, but clinically significant, JAK2 mutations.
Sample Required
Whole Blood (WB) or Bone Marrow Aspirate (BMA) in EDTA Vacutainer
(A sample of whole blood or bone marrow aspirate collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the sample for DNA extraction.)
Preparation
No specific preparation is typically required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood or bone marrow sample collection. * No Special Timing: Blood or bone... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.