Molecular Genetic Sickle Cell Anaemia Mutation Analysis - RT-PCR
Test Code
MOG09
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Reverse Transcription Polymerase Chain Reaction) / Allele-Specific PCR
The Molecular Genetic Sickle Cell Anemia Mutation Analysis primarily utilizes Allele-Specific PCR (AS-PCR), a highly sensitive and specific variation of PCR (Polymerase Chain Reaction). While the prompt mentions RT-PCR, for DNA-based point mutations, standard PCR is commonly applied directly to DNA. AS-PCR is employed to directly detect the HbS mutation (beta-globin gene Glu6Val or E6V). This method uses primers specifically designed to bind only to the mutated DNA sequence, allowing for selective amplification of the mutated allele. The detection of a specific amplified product confirms the presence of the sickle cell mutation. This technique provides highly accurate qualitative detection, determining the presence or absence of this key genetic alteration, which is crucial for diagnosis and carrier screening of sickle cell anemia and sickle cell trait.
Sample Required
Whole Blood (WB) in EDTA Vacutainer
(A sample of whole blood collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the blood sample for DNA extraction.)
Preparation
No specific preparation is typically required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood sample collection. * No Special Timing: Blood can be drawn at any ti... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.