Molecular Genetic JAK2 - V617F Mutation Detection by RT-PCR
Test Code
MOG06
Also Known As
No data available
Test Parameters Included
No data available
Department
MOLECULAR GENETICS
Methodology
RT-PCR (Reverse Transcription Polymerase Chain Reaction) / Allele-Specific PCR
The Molecular Genetic JAK2 - V617F Mutation Detection test primarily utilizes Allele-Specific PCR (AS-PCR), a variation of PCR (Polymerase Chain Reaction), which may be integrated into an RT-PCR workflow if RNA is initially extracted and then reverse transcribed to cDNA (though typically DNA is directly used for point mutations). This highly sensitive molecular technique is designed to specifically detect the V617F point mutation in the JAK2 gene. AS-PCR uses primers that are specifically designed to bind only to the mutated DNA sequence, allowing for selective amplification of the mutated allele even when present at low levels. The amplification of a specific product indicates the presence of the V617F mutation. This method provides highly accurate qualitative detection, determining the presence or absence of this key somatic mutation associated with myeloproliferative neoplasms.
Sample Required
WB/BMA EDTA Whole Blood (WB) or Bone Marrow Aspirate (BMA) in EDTA Vacutainer
(A sample of whole blood or bone marrow aspirate collected in an EDTA vacutainer is required for this test. EDTA acts as an anticoagulant to preserve the sample for DNA extraction.)
Preparation
No specific preparation is typically required for this genetic test. * No Fasting Required: Fasting is not necessary for this blood or bone marrow sample collection. * No Special Timing: Blood or bone... Read more
Schedule Report
3 Days
Emergency Report
Yes
Frequently Asked Questions
No data available
Test Description
- High-end laboratory & medical equipment.
- Outstanding doctor team.
- Personalized care & high-quality service.
- Tailormade health screening packages.